Join us to learn about and engage with an array of campus research service providers this summer. While you are thinking about writing articles and grant proposals or thinking up new programs, it's also a good time to refresh our memories about campus services that may enhance those research and scholarly activities.
 

Each service unit will provide a five- to ten-minute recorded video highlighting their services. This recording will be provided below approximately one week ahead of a 30-minute Q&A session via Zoom.  
 

See the details below regarding the upcoming Spotlight sessions that will take place on Wednesdays from 10am-10:30am.

 

Flow Cytometry Facility (Wednesday, June 3 @ 10am-10:30am)

The Flow Cytometry Facility provides laser-based instrumentation for the study of biological systems using single-cell suspensions tagged with fluorescent markers.  Facility services include: high speed cell sorting/cloning, multi-parameter (multi-color) analysis, multiplexed cytokine analysis, cell cycle analysis, consultation for experiment design, training and assisting investigators in the use of software programs and bench-top instruments, data analysis, cell preparation optimization, staining protocols, online instrument scheduling and data storage on a dedicated server.
Presenter: Heath Vignes, Core Facility Research Manager

Register for the Q&A Session here: www.eventbrite.com/e/106396099598

Video: https://www.youtube.com/watch?v=55UOmN5m5g0&list=PLNZ27x5yQwbYJWdinVuRgODml8DmLbswb

Electron Spin Resonance Facility (Wednesday, June 10 @ 10am-10:30am)- POSTPONED to Wednesday, July 8 @ 10-10:30am

The ESR Facility addresses research at the interface of free radical biology and medicine. We provide the expertise and instrumentation to pursue research questions dealing with oxygen free radicals, singlet oxygen, nitric oxide and the array of related oxidants and antioxidants that influence the overall redox environment of cells, tissues, and whole organisms. Our goal is to advance the fundamental understanding of free radical biology to improve human health through innovative approaches in the prevention and treatment of disease. Example services include: 1) EPR spectroscopy to detect free radicals, directly or with spin trapping approaches or redox active transition metals, e.g. Fe3+ and Cu2+; 2) Sievers NOA280i for the detection of footprints of nitric oxide, e.g. nitrite and nitrate; 3) Clark Electrode for the detection of oxygen uptake by chemical, biochemical, and cellular systems; 4) Seahorse XF96 to quantitatively determine (and dissect) rates of production of ATP by cells; 5) Quantitative assays for a wide range of species, e.g. vitamin C, superoxide, GPx4, and other species of interest in the field of redox biology. 
Presenters: Garry Buettner, Director and Brett Wagner, Senior Research Assistant

Register for the Q&A session here: www.eventbrite.com/e/106397860866

Video: https://youtu.be/ajmXrsehojk

UI Pharmaceuticals (Wednesday, June 17 @ 10am-10:30am)

UIP is an FDA-registered pharmaceutical manufacturing and testing facility. UIP provides contract pharmaceutical services including pre-formulation studies, formulation development, clinical supply manufacturing, small scale commercial manufacturing, analytical method development and validation, routine quality control analysis, and stability studies. UIP is licensed by the DEA to handle controlled substances (Schedules I - V) and capable of handling potent and cytotoxic compounds.  UIP produces sterile injectable (including lyophilized products) and non-sterile liquid, solid oral, and semisolid dosage forms.
Presenter: Randy Yeates, Director

Register for the Q&A Session here: www.eventbrite.com/e/106398430570

Video: https://www.youtube.com/watch?v=zaHSf8AKWsI&feature=youtu.be

High Resolution Mass Spectrometry Facility (Wednesday, June 24 @ 10-10:30am)

The High Resolution Mass Spectrometry Facility (HRMSF) provides high resolution mass spectrometry services for researchers at the University of Iowa and across the United States.  Using high resolution mass spectrometers, we can obtain accurate mass measurements to determine the elemental compositions of new synthetic molecules, metabolites, and natural products.  We can also perform tandem mass spectrometry (MS/MS) experiments, which are used to assist in the structure determination of unknown molecules and quantitative analysis at trace levels.  We have four mass spectrometers available (both GC-MS and LC-MS) for on-campus researchers.  Open access instruments are available to those on-campus who have been trained by the HRMSF staff.  Presently we serve researchers in over 35 research groups from six different colleges within the University of Iowa.
Presenter: Lynn Teesch, Director

Register for the Q&A Session here: www.eventbrite.com/e/106398723446

Video: https://www.youtube.com/watch?v=h3vTOxoS9to&feature=youtu.be

Materials Analysis, Testing, and Fabrication [MATFab] Facility (Wednesday, July 1 @ 10-10:30am)

The Materials Analysis, Testing, and Fabrication (MATFab) Facility brings together cutting-edge instruments for the physical sciences and engineering. We offer a diverse set of analytical instrumentation for elemental and chemical analysis, imaging, and micro and nano fabrication. Our facility also offers training to researchers and graduate students on our equipment, giving the opportunity to learn valuable techniques on equipment they would not otherwise have in their own research labs.
Presenters: Phil Pagano, Core Facilities Research Specialist and Connor Grierson, Core Facilities Research Prof

Register for the Q&A Session here: https://www.eventbrite.com/e/106438833416

Video: https://youtu.be/XLSrh4Mtwkc

The following will be formatted as one-hour Zoom session that will feature ten-minute service overviews by three research core facilities followed by a thirty-minute Q&A breakout sessions with each unit.
 

Wednesday, July 29 @ 10-11am

Metabolic Phenotyping Core:

The Metabolic Phenotyping Core provides investigators specialized and non-invasive metabolic assays that are essential in phenotyping mouse and other animal models with diabetes, its complications, obesity, and related metabolic disorders. The central services of the core include: 1) Determination of whole animal energy expenditure using Metabolic Chambers: Promethion (Sable Systems International) and CLAMS (Comprehensive Lab Animal Monitoring System, Columbus Instruments). This is a non-invasive measurement of food intake, energy expenditure, respiratory exchange ratio and physical activity. 2) Measurement of whole body composition with a Bruker MiniSpec in mice and rats. 3) Hyperinsulinemic-euglycemic clamp experiments to assess in vivo insulin action, insulin signaling, and glucose metabolism in awake mice. 4)Hyperglycemic clamp experiments to assess in vivo pancreatic b-cell function (i.e., glucose-induced insulin secretion) and the effect of hyperglycemia on glucose metabolism (i.e., glucose toxicity). 5)Mitochondrial bioenergetics: tissue/cellular/isolated mitochondria oxygen consumption using the XF-24 Extracellular Flux Analyzer; mitochondrial respirometry for tissue (permeabilized mouse heart and soleus) and isolated mitochondria with the O2K from OROBOROS. 6) Glucose and insulin tolerance tests. Specific equipment available at the Metabolic Phenotyping Core includes: a Seahorse XFe-24 analyzer, O2K from OROBOROS, two Bruker Minispecs, and Metabolic Chambers.
Presenters: Jamie Soto; Technical Director and Wojciech Grzesik; Clamps Expert

CCOM Nuclear Magnetic Resonance Facility:

The CCOM NMR Facility supports the biomedical research community with three high field (500 MHz, 600 MHz and 800 MHz) NMR spectrometers capable of probing the structure and dynamics of biomolecules using a wide range of techniques. Full spectroscopic and interpretive services are offered, as well as assistance and training for researchers who wish to perform their own experiments. Example NMR spectroscopy projects include: 1) Determination of three-dimensional structures for proteins in solution; 2) Observation of the fate of a chemical species as they are processed by a cell culture; 3) Identification of and quantification of components in a mixture, and 4) Characterization of ligand binding and aggregation in a solution.
Presenters: Liping Yu; Director and Chris Ptak; Core Facilities Research Specialist

UI High Throughput Screening Facility:

UIHTS is a high throughput platform that integrates robotics, detection systems, chemical/biologics libraries, and data/image analysis. UIHTS enables highly flexible and scalable screening approaches, not only to foster hit and lead generation for drug discovery and development through screening of large chemical/biologics libraries; but also to facilitate molecular probe discovery for mechanism of action (MOA) studies of chemical biology through screening of focused intellectually-designed compound/gRNA collections.
Presenter: Meng Wu; Director

Register for the July 29 session here: https://www.eventbrite.com/e/research-services-spotlight-series-tickets-111678675926

 

Questions

For questions, please contact vpr-rdo@uiowa.edu

 

Individuals with disabilities are encouraged to attend all University of Iowa–sponsored events. If you are a person with a disability who requires a reasonable accommodation in order to participate in this program, please contact Britt Ryan in advance at 319-335-9575 or vpr-rdo@uiowa.edu